CYP24A1

Cytochrome P450 family 24 subfamily A member 1 (abbreviated CYP24A1) is a member of the cytochrome P450 superfamily of enzymes encoded by the CYP24A1 gene. It is a mitochondrial monooxygenase which catalyzes reactions including 24-hydroxylation of calcitriol (1,25-dihydroxyvitamin D₃). It has also been identified as vitamin D3 24-hydroxylase.(EC 1.14.15.16)

Function

CYP24A1 is an enzyme expressed in the mitochondrion of humans and other species. It catalyzes hydroxylation reactions which lead to the degradation of 1,25-dihydroxyvitamin D₃, the physiologically active form of vitamin D. Hydroxylation of the side chain produces calcitroic acid and other metabolites which are excreted in bile.

CYP24A1 was identified in the early 1970s and was first thought to be involved in vitamin D metabolism as the renal 25-hydroxyvitamin D3-24-hydroxylase, modifying calcifediol (25-hydroxyvitamin D) to produce 24,25-dihydroxycholecalciferol (24,25-dihydroxyvitamin D). Subsequent studies using recombinant CYP24A1 showed that it could also catalyze multiple other hydroxylation reactions at the side chain carbons known as C-24 and C-23 in both 25-OH-D₃ and the active hormonal form, 1,25-(OH)₂D₃. It is now considered responsible for the entire five-step, 24-oxidation pathway from 1,25-(OH)₂D₃ producing calcitroic acid.

CYP24A1 also is able to catalyze another pathway which starts with 23-hydroxylation of 1,25-(OH)₂D₃ and culminates in 1,25-(OH)₂D₃-26,23-lactone.

The side chains of the ergocalciferol (vitamin D₂) derivatives, 25-OH-D₂ and 1,25-(OH)₂D₂, are also hydroxylated by CYP24A1.

The structure of CYP24A1 is highly conserved between different species although the balance of functions can differ. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

This enzyme plays an important role in calcium homeostasis and the vitamin D endocrine system through its regulation of the level of vitamin D₃.

Interactive pathway map

Click on genes, proteins and metabolites below to link to respective articles.

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Vitamin D Synthesis Pathway (view / edit)

Regulation

CYP24A1 is expressed in tissues which are considered targets for vitamin D, including kidney, intestine and bone. Transcription of the CYP24A1 gene is markedly inducible by 1,25-(OH)₂D₃ binding to the vitamin D receptor. The gene has a strong, positive vitamin D response element in the promoter. Through regulation of CYP24A1 expression, a negative feedback control system is created to limit the effects of 1,25-(OH)₂D₃.

PTH and FGF23 also regulate CYP24A1 gene expression. Additionally, it is translationally regulated via IRES within the 5'UTR, which is responsive to an inflammatory environment.

Clinical relevance

Abnormal functioning CYP24A1 is thought to be one of the causes of severe infantile hypercalcemia. However, increasingly patients are also being diagnosed in adulthood, often when they present with hypercalcaemia. Patients with mutations of the CYP24A1 gene have elevated serum calcium concentrations, elevated serum 1,25-(OH)₂D, suppressed PTH concentrations, hypercalciuria, nephrocalcinosis, nephrolithiasis, and sometimes reduced bone density. Variations in the gene may also be found in people with renal stones.

External links

• Human CYP24A1 genome location and CYP24A1 gene details page in the UCSC Genome Browser.