TRiC (complex)
T-complex protein Ring Complex (TRiC), otherwise known as Chaperonin Containing TCP-1 (CCT),[a] is a multiprotein complex and the chaperonin of eukaryotic cells. Like the bacterial GroEL, the TRiC complex aids in the folding of ~10% of the proteome, and actin and tubulin are some of its best known substrates.[2][3] TRiC is an example of a biological machine that folds substrates within the central cavity of its barrel-like assembly using the energy from ATP hydrolysis.
Subunits
[edit | edit source]The human TRiC complex is formed by two rings containing 8 similar but non-identical subunits, each with molecular weights of ~60 kDa. The two rings are stacked in an asymmetrical fashion, forming a barrel-like structure with a molecular weight of ~1 MDa.[2][3]
| Subunit | MW (kDa)[A] | Features |
|---|---|---|
| TCP1 (CCT1/α) | 60 | |
| CCT2 (β) | 57 | |
| CCT3 (γ) | 61 | |
| CCT4 (δ) | 58 | |
| CCT5 (ε) | 60 | |
| CCT6 (ζ) | 58 | Two copies in human genome, CCT6A and CCT6B. |
| CCT7 (η) | 59 | |
| CCT8 (θ) | 60 |
A Molecular weight of human subunits.
Counterclockwise from the exterior, each ring is made of the subunits in the following order: 6-8-7-5-2-4-1-3.[4]
Evolution
[edit | edit source] | This section is missing information about phylogeny (template) and evolutionary trajectory (pic). (December 2020) |
The CCT evolved from the archaeal thermosome ~2Gya, with the two subunits diversifying into multiple units. The CCT changed from having one type of subunit, to having two, three, five, and finally eight types.[4]: fig. 4
See also
[edit | edit source]Notes
[edit | edit source]- ^ The term "TCP-1" is variously expanded as "T-complex protein 1" and "tailless complex polypeptide 1". The "T-complex" is the same as tailless complex, a CCT locus associated with tail length in mice.
References
[edit | edit source]- ^ Lua error in Module:Citation/CS1/Configuration at line 2172: attempt to index field '?' (a nil value).
- ^ a b Lua error in Module:Citation/CS1/Configuration at line 2172: attempt to index field '?' (a nil value).
- ^ a b Lua error in Module:Citation/CS1/Configuration at line 2172: attempt to index field '?' (a nil value).
- ^ a b Lua error in Module:Citation/CS1/Configuration at line 2172: attempt to index field '?' (a nil value).
Lua error in mw.title.lua at line 392: bad argument #2 to 'title.new' (unrecognized namespace name 'Portal').