DNA beta-glucosyltransferase
| DNA beta-glucosyltransferase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 2.4.1.27 | ||||||||
| CAS no. | 9030-14-2 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
In enzymology, a DNA beta-glucosyltransferase (EC 2.4.1.27) is an enzyme that catalyzes the chemical reaction in which a beta-D-glucosyl residue is transferred from UDP-glucose to an hydroxymethylcytosine residue in DNA. It is analogous to the enzyme DNA alpha-glucosyltransferase.
This enzyme belongs to the family of glycosyltransferases, specifically the hexosyltransferases. The systematic name of this enzyme class is UDP-glucose:DNA beta-D-glucosyltransferase. Other names in common use include T4-HMC-beta-glucosyl transferase, T4-beta-glucosyl transferase, T4 phage beta-glucosyltransferase, UDP glucose-DNA beta-glucosyltransferase, and uridine diphosphoglucose-deoxyribonucleate beta-glucosyltransferase.
Structural studies
[edit | edit source]As of late 2007, 20 structures have been solved for this class of enzymes, with PDB accession codes 1BGT, 1BGU, 1C3J, 1IXY, 1J39, 1JEJ, 1JG6, 1JG7, 1JIU, 1JIV, 1JIX, 1M5R, 1NVK, 1NZD, 1NZF, 1QKJ, 1SXP, 1SXQ, 2BGT, and 2BGU.
Bacteriophage T4 beta-glucosyltransferase
[edit | edit source]| T4-Gluco-transf | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| File:PDB 1m5r EBI.jpg ternary complex of t4 phage bgt with udp and a 13 mer dna duplex | |||||||||
| Identifiers | |||||||||
| Symbol | T4-Gluco-transf | ||||||||
| Pfam | PF09198 | ||||||||
| InterPro | IPR015281 | ||||||||
| SCOP2 | 1jix / SCOPe / SUPFAM | ||||||||
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In molecular biology, Bacteriophage T4 beta-glucosyltransferase refers to a protein domain found in a virus of Escherichia coli named bacteriophage T4. Members of this family are enzymes encoded by bacteriophage T4, which modify DNA by transferring glucose from uridine diphosphoglucose to 5-hydroxymethyl cytosine bases of phage T4 DNA.[1]
Function
[edit | edit source]Beta-glucosyltransferase is an enzyme, or more specifically an inverting glycosyltransferase (GT). In other words, it transfers glucose from uridine diphospho-glucose (UDPglucose) to an acceptor, modified DNA through beta-Glycosidic bond. The role of the enzyme is to protect the infecting viral DNA from the bacteria's restriction enzymes. Glucosylation prevents the virus DNA from being cut up. Furthermore, glucosylation may aid gene expression of the bacteriophage by influencing transcription.[2][3][4]
Structure
[edit | edit source]This structure has both alpha helices and beta strands.[2]
References
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