Radiobinding assay
A radiobinding assay is a method of detecting and quantifying antibodies targeted toward a specific antigen. As such, it can be seen as the inverse of radioimmunoassay, which quantifies an antigen by use of corresponding antibodies.
Technique
[edit | edit source]The corresponding antigen is radiolabeled and mixed with the fluid that may contain the antibody, such as blood serum from a person. Presence of antibodies causes precipitation of antibody-antigen complexes that can be collected by centrifugation into pellets. The amount of antibody is proportional to the radioactivity of the pellet, as determined by gamma counting.[1]
Uses
[edit | edit source]It is used to detect most autoantibodies seen in latent autoimmune diabetes.[2]
References
[edit | edit source]- ^ Anti-dsDNA [I-125] Radiobinding Assay Kit[permanent dead link] At PerkinElmer Life Sciences, Inc. Retrieved Jan 2011
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