PtK2 cells
PtK2 cells are a cell line derived from male long-nosed potoroo (Potorous tridactylis) epithelial kidney cells.[1] This cell line is used for a variety of applications in biomedical research but is particularly popular as a model for mitosis.[2]
Origin
[edit | edit source]The PtK2 cell line was established by Kirsten Walen and Spencer Brown in 1962.[3] Cells from the rat kangaroo were selected as the source of a cell line because this species has only a small number of chromosomes and these chromosomes are easily visualized under a microscope; mitosis becomes easy to observe, and the structural aspects of cell division can be closely examined.[4]
Characteristics
[edit | edit source]PtK2 cells are relatively large, and when grown in a monolayer, stay flat throughout the cell cycle - unlike many cells that round up during mitosis.[5] PtK2 cells are resistant to adenovirus 5, coxsackievirus B5, and poliovirus 2. They are susceptible to coxsackievirus A9, herpes simplex, vaccinia, and vesicular stomatitis (Ogden strain).[6] PtK2 cells contain intermediate filaments composed of Keratin.[7]
References
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