Pierce Protein Assay
This article relies largely or entirely on a single source. (July 2015) |
The Pierce Protein Assay is a method of protein quantification. It provides quick estimation of the protein amount in a given sample.[1]
Protocol
[edit | edit source]The assay is separated into three main parts: preparation of the Diluted Albumin (BSA) Standards, preparation of the bicinchoninic acid (BCA) working reagent, and quantification of proteins (using either test tube or microplate procedure).
Advantages and disadvantages
[edit | edit source]Advantages
[edit | edit source]This method is able to detect as low as 25 μg/ml and up to 2000 μg/ml of protein in a 65 ul sample, using standard protocol. This method may be preferred for samples containing detergents or other reducing agents. This method has a fast detection speed and low protein-to-protein variability in comparison to the BCA or Coomassie (Bradford) Assays. This method has a stable end point.
Disadvantages
[edit | edit source]This method has greater protein-to-protein variability than the BCA Assay.
References
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