Pentafluorophenyl esters

Generic structure of a pentafluorophenyl ester

Pentafluorophenyl (PFP) esters are chemical compounds with the generic formula RC(O)OC₆F₅. They are active esters derived from pentafluorophenol (HOC₆F₅).

PFP esters are useful for attaching fluorophores such as fluorescein^[1] or haptens^[2] to primary amines in biomolecules. They also are valuable in laboratory peptide synthesis. Pentafluorophenyl esters produce amide bonds as effectively as succinimidyl esters and various similar agents do, but PFP esters are particularly useful because they are less susceptible to spontaneous hydrolysis during conjugation reactions.^[3]

File:Pentafluorophenylester formation of 6-carboxyfluoroscein.svg

Scheme of pentafluorophenylester formation of 6-carboxyfluoroscein

References

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Pentafluorophenyl esters

References

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