Optimized Multicolor Immunofluorescence Panel

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Optimized Multicolor Immunofluorescence Panel, abbreviation OMIP, was a series of standardized multi-color flow cytometry panel launched by International Society for Advancement of Cytometry (ISAC), published in the form of columns in Cytometry Part A.[1][2][3] The first two OMIP articles were published in September 2010 issue of this journal.[4]

Purpose

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The goals of OMIPs are:

  1. to alleviate the development time for researchers who wish to use the same (or highly similar) panels
  2. to provide a starting point for the creation of novel OMIPs, and
  3. to provide a mechanism for attribution to the developers of the panel via citation of the publication.[1]

List

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No. No. of colors Purpose Species Cell types Paper Year Cytometry
OMIP-001 16 T-cell cytokine production after in vitro stimulation and phenotyping of cytokine-producing T-cells Human PBMC [5][4] 2010 BD LSR II
OMIP-002 16 Specificity and phenotype of CD8+ T-cells Human PBMC, bone marrow mononuclear cells [6][4] 2010 BD LSR II
OMIP-003 12 Phenotyping memory B cells human PBMC, bone marrow, and tonsil mononuclear cell [7] 2011 BD LSR II
OMIP-004 15 Determine Treg levels and to provide an in depth characterization of Tregs Human PBMC, ACK-lysed blood [8] 2012 BD LSRFortessa
OMIP-005 11 T cell cytokine production and memory phenotype Rhesus macaque PBMC [9] 2012 BD LSR II
OMIP-006 9 Enumerating and phenotyping of T regulatory cells and subsets Human PBMC [10] 2012 BD LSR II
OMIP-007 14 Characterize the phenotype, maturation, and activation of NK cells Human PBMC [11] 2012
OMIP-008 11 T cell cytokine production following in vitro stimulation Human PBMC, T cell clones/lines, tumor-resident T cell lines [12] 2012
OMIP-009 10 phenotype and function of CD4+ and CD8+ T-cells Human PBMC [13] 2012
OMIP-010 10 immunophenotyping of small hematopoietic cell Human Cerebrospinal fluid, bone marrow, peripheral blood [14] 2012
OMIP-011 8 Characterization of CECs in peripheral blood Human Whole blood, RBC-lysed and washed [15] 2012
OMIP-012 9 Human leukocyte reconstitution and phenotype Humanized mouse Whole blood, Ficoll-separated PBMC, splenocytes [16] 2012
OMIP-013 13 T-cell differentiation Human PBMC [17] 2012
OMIP-014 12 T-cell cytokine production and function after in vitro stimulation Human Cryopreserved PBMC [18] 2012
OMIP-015 13 T-reg characterization and T-cell activation Human PBMC [19] 2013
OMIP-016 10 Quality and phenotype of antigen-specific CD4 and CD8 T cells Cynomolgus macaque or Human Fresh or cryopreserved PBMCs [20] 2013
OMIP-017 13 CD4+ helper T-cell subsets (Th1, Th2, Th9, Th17, Th17Th1, Th22 and TFH) Human PBMC [21] 2013
OMIP-018 15 Phenotype of CD4 T cell subsets via chemokine receptor expression Human Fresh PBMC, Bone marrow mononuclear cells [22][23] 2013
OMIP-019 14 γδT-cells, iNKT-cells, haematopoietic precursors Human PBMC [24] 2013
OMIP-020 12 γδ T-cell differentiation phenotype Human PBMC [25] 2014
OMIP-021 14 NKT cells, γδ T-cells, MAIT cells, Memory T cell subsets Human PBMC [26] 2014
OMIP-022 14 T-cell phenotyping, memory categorization, cytokine production, and function following in vitro stimulation Human Cryopreserved PBMC (adult and infant) [27] 2014
OMIP-023 10 In-depth phenotyping of the main leukocyte subtypes in one run Human Fresh EDTA-treated peripheral blood, RBC-lysed and Fresh EDTA-treated Cord-blood [28] 2014
OMIP-024 18 Characterize the phenotype of PBMC leukocyte subsets Human Cryopreserved PBMC from adults and infants [29] 2014
OMIP-025 16 Characterization of antigen-specific T cells, TFH-like cells and NK cells Human Cryopreserved PBMC [30] 2014
OMIP-026 14 Phenotype of B and plasma cells Rhesus macaque, African green monkey Fresh whole blood, PBMCs, mononuclear cells of bone marrow, lymph node, spleen, tonsil [31] 2015
OMIP-027
OMIP-028
OMIP-029
OMIP-030
OMIP-031
OMIP-032 [32]
OMIP-033
OMIP-034
OMIP-035
OMIP-036
OMIP-037
OMIP-038
OMIP-039
OMIP-040
OMIP-041
OMIP-042
OMIP-043
OMIP-044
OMIP-045
OMIP-046
OMIP-047
OMIP-048
OMIP-049 19 Subset analysis of hematopoiesis and hematopoietic disease Human Any source containing human myeloid cells [33] 2018 BD LSR II
OMIP-050 28 [34]
OMIP-051
OMIP-052
OMIP-053
OMIP-054
OMIP-055
OMIP-056
OMIP-057
OMIP-058
OMIP-059
OMIP-060
OMIP-061
OMIP-062 14 Myeloid and innate/adaptive lymphoid comprehensive immunophenotyping Human Whole peripheral blood, cord blood, PBMCs, nasal aspirate, and tracheal aspirate samples [35] 2019 BD LSRFortessa
OMIP-063
OMIP-064
OMIP-065
OMIP-066
OMIP-067
OMIP-068
OMIP-069
OMIP-070
OMIP-071
OMIP-072
OMIP-073
OMIP-074
OMIP-075
OMIP-076
OMIP-077
OMIP-078
OMIP-079
OMIP-080
OMIP-081
OMIP-082 25
OMIP-083 18
OMIP-084 28
OMIP-085 8
OMIP-086 22
OMIP-087
OMIP-088
OMIP-089 8
OMIP-090 18
OMIP-091 27
OMIP-092 6
OMIP-093 41
OMIP-094 24
OMIP-095
OMIP-096
OMIP-097
OMIP-098
OMIP-099
OMIP-100
OMIP-101 27 Broad immunophenotyping of leukocytes including myeloid (neutrophils, monocytes, mDCs) and lymphoid cell lineages (conventional T cells, DURT cells, γδT cells, B cells, NK cells, ILCs) Human Fresh or fixed, cryopreserved whole blood [36] 2024 BD FACSymphony A5

References

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