Optimal cutting temperature compound

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File:Tissue for frozen section in cryostat.JPG
Frozen section procedure: tissue embedded in optimal cutting temperature compound, mounted on a chuck in a cryostat and ready for section production

Optimal cutting temperature (OCT) compound is used to embed tissue samples prior to frozen sectioning on a microtome-cryostat. This process is undertaken so as to mount slices (sections) of a sample onto slides for analysis.

Components

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From MSDS:[1]

  • 5–15% polyvinyl alcohol
  • 1–10% polyethylene glycol
  • 75–95% non-reactive ingredients

See also

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References

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Further reading

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