MOPS
| Names | |
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| Preferred IUPAC name
3-(Morpholin-4-yl)propane-1-sulfonic acid | |
| Other names
3-(N-Morpholino)propanesulfonic acid,
3-Morpholinopropanesulfonic acid, 3-N-Morpholino propansulfonic acid, 4-Morpholinepropanesulfonic acid | |
| Identifiers | |
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3D model (JSmol)
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| ChemSpider | |
| ECHA InfoCard | Lua error in Module:Wikidata at line 880: attempt to index field 'wikibase' (a nil value). Lua error in Module:Wikidata at line 880: attempt to index field 'wikibase' (a nil value).Lua error in Module:EditAtWikidata at line 29: attempt to index field 'wikibase' (a nil value). |
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PubChem CID
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| UNII | |
CompTox Dashboard (EPA)
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| Properties | |
| C7H15NO4S | |
| Molar mass | 209.26 g·mol−1 |
| Hazards | |
| GHS labelling: | |
| GHS07: Exclamation mark | |
| Warning | |
| H315, H319, H335 | |
| P261, P264, P271, P280, P302+P352, P304+P340, P305+P351+P338, P312, P321, P332+P313, P337+P313, P362, P403+P233, P405, P501 | |
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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MOPS (3-(N-morpholino)propanesulfonic acid) is a buffer introduced in the 1960s, one of the twenty Good's buffers. It is a structural analog to MES,[1] and like MES, its structure contains a morpholine ring. HEPES is a similar pH buffering compound that contains a piperazine ring. With a pKa of 7.20, MOPS is an excellent buffer for many biological systems at near-neutral pH.
Applications
[edit | edit source]MOPS is frequently used as a buffering agent in biology and biochemistry. It has been tested and recommended for polyacrylamide gel electrophoresis.[2] Usage above 20 mM in mammalian cell culture work is not recommended.[3] MOPS buffer solutions become discolored (yellow) over time, but reportedly slight discoloration does not significantly affect the buffering characteristics.[4]
See also
[edit | edit source]References
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External links
[edit | edit source]- Sigma Aldrich Buffer Calculator - Useful tool to calculate weight, volume, or concentration from molecular weight.
- Recipe for MOPS buffer on OpenWetWare