Immunoscreening
Immunoscreening is a method of biotechnology used to detect a polypeptide produced from a cloned gene. The term encompasses several different techniques designed for protein identification, such as Western blotting, using recombinant DNA, and analyzing antibody-peptide interactions.[1]
Clones are screened for the presence of the gene product: the resulting protein. This strategy requires first that a gene library is implemented in an expression vector, and that antiserum to the protein is available. Radioactivity or an enzyme is coupled generally with the secondary antibody. The radioactivity/enzyme linked secondary antibody can be purchased commercially and can detect different antigens. In commercial diagnostics labs, labelled primary antibodies are also used.[2] The antigen-antibody interaction is used in the immunoscreening of several diseases.[3]
See also
[edit | edit source]References
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- ^ Mukerjee S, McKnight ME, Glassy MC. Immunoscreening protocols for the identification of clinically useful antibodies and antigens. Expert Opin Investig Drugs. 1998 Mar;7(3):373-89. doi: 10.1517/13543784.7.3.373. PMID 15991979.
