EIF1
Lua error in Module:Infobox_gene at line 53: attempt to index field 'wikibase' (a nil value). Eukaryotic translation initiation factor 1 (eIF1) is a protein that in humans is encoded by the EIF1 gene. It is related to yeast SUI1.[1][2][3]
eIF1 interacts with the eukaryotic small (40S) ribosomal subunit and eIF3, and is a component of the 43S preinitiation complex (PIC).[4] eIF1 and eIF1A bind cooperatively to the 40S to stabilize an "open" conformation of the preinitiation complex (PIC) during eukaryotic translation initiation.[4] eIF1 binds to a region near the ribosomal P-site in the 40S subunit and functions in a manner similar to the structurally related bacterial counterpart IF3.[5]
Structure
[edit | edit source]eIF1's structure was first determined in 1999 by solution-state NMR spectroscopy, which revealed that it consists of a five-stranded beta-sheet which is sided by two alpha-helices.[6] Crystallographic experiments showed that eIF1 is located at the P-site of the small ribosomal subunit, binding to the 18S rRNA with a basic surface.[7] To date, a number of cryo-EM structures have been solved that include eIF1 in the context of various translation initiation complexes.[8][9][10]
Function
[edit | edit source]In eukaryotic cells, translation initiation on an mRNA involves scanning of the mRNA by the 43S pre-initiation complex in search of the translation initiation start codon.[11] Accurate identification of the start codon is very important, as other translation start sites may lead to the production of defect proteins. A codon is detected as a start codon by interaction with the tRNA's anticodon that is positioned in the P-site of the small ribosomal subunit, which leads to closing of the pre-initiation complex.[12] eIF1 is positioned on the small ribosomal subunit such that it blocks the closure of the pre-initiation complex. It thereby aids in selecting the correct start codon, since only the correct codon-anticodon interaction provides enough energy to displace eIF1 and thus close the pre-initiation complex.[13]
See also
[edit | edit source]References
[edit | edit source]- ^ Lua error in Module:Citation/CS1/Configuration at line 2172: attempt to index field '?' (a nil value).
- ^ Lua error in Module:Citation/CS1/Configuration at line 2172: attempt to index field '?' (a nil value).
- ^ Lua error in Module:Citation/CS1/Configuration at line 2172: attempt to index field '?' (a nil value).
- ^ a b Lua error in Module:Citation/CS1/Configuration at line 2172: attempt to index field '?' (a nil value).
- ^ Lua error in Module:Citation/CS1/Configuration at line 2172: attempt to index field '?' (a nil value).
- ^ Lua error in Module:Citation/CS1/Configuration at line 2172: attempt to index field '?' (a nil value).
- ^ Lua error in Module:Citation/CS1/Configuration at line 2172: attempt to index field '?' (a nil value).
- ^ Lua error in Module:Citation/CS1/Configuration at line 2172: attempt to index field '?' (a nil value).
- ^ Lua error in Module:Citation/CS1/Configuration at line 2172: attempt to index field '?' (a nil value).
- ^ Lua error in Module:Citation/CS1/Configuration at line 2172: attempt to index field '?' (a nil value).
- ^ Lua error in Module:Citation/CS1/Configuration at line 2172: attempt to index field '?' (a nil value).
- ^ Lua error in Module:Citation/CS1/Configuration at line 2172: attempt to index field '?' (a nil value).
- ^ Lua error in Module:Citation/CS1/Configuration at line 2172: attempt to index field '?' (a nil value).
- ^ Lua error in Module:Citation/CS1/Configuration at line 2172: attempt to index field '?' (a nil value).
Further reading
[edit | edit source]- Lua error in Module:Citation/CS1/Configuration at line 2172: attempt to index field '?' (a nil value).
- Lua error in Module:Citation/CS1/Configuration at line 2172: attempt to index field '?' (a nil value).
- Lua error in Module:Citation/CS1/Configuration at line 2172: attempt to index field '?' (a nil value).
- Lua error in Module:Citation/CS1/Configuration at line 2172: attempt to index field '?' (a nil value).
External links
[edit | edit source]- EIF1 human gene location in the UCSC Genome Browser.
- EIF1 human gene details in the UCSC Genome Browser.
- Cap-dependent translation initiation from Nature Reviews Microbiology. A good image and overview of the function of initiation factors
- Overview of all the structural information available in the PDB for UniProt: P41567 (Eukaryotic translation initiation factor 1) at the PDBe-KB.
