EDANS

EDANS

Names
Preferred IUPAC name 5-[(2-Aminoethyl)amino]naphthalene-1-sulfonic acid
Other names EDANS
Identifiers
CAS Number	50402-56-7 Y
3D model (JSmol)	Interactive image
ChemSpider	83355 ☒N
ECHA InfoCard
E number
PubChem CID	92329
UNII	3JBY896YZF Y
CompTox Dashboard (EPA)	{{#property:P3117}}
InChI InChI=1S/C12H14N2O3S/c13-7-8-14-11-5-1-4-10-9(11)3-2-6-12(10)18(15,16)17/h1-6,14H,7-8,13H2,(H,15,16,17) ☒N Key: SJQRQOKXQKVJGJ-UHFFFAOYSA-N ☒N InChI=1/C12H14N2O3S/c13-7-8-14-11-5-1-4-10-9(11)3-2-6-12(10)18(15,16)17/h1-6,14H,7-8,13H2,(H,15,16,17) Key: SJQRQOKXQKVJGJ-UHFFFAOYAN
SMILES c1cc2c(cccc2S(=O)(=O)O)c(c1)NCCN
Properties
Chemical formula	C12H14N2O3S
Molar mass	266.32
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa). ☒N verify (what is Y☒N ?) Infobox references

EDANS (5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid) is a donor for FRET-based nucleic acid probes and protease substrates. EDANS is often paired with DABCYL or DABSYL. The combination can be used in enzyme assays. When the two compounds are in close proximity, most of the energy emitted from EDANS will be quenched by DABCYL. However, if the compounds are separated (for example, by substrate cleavage) EDANS will fluoresce, giving an indication of enzyme presence.

See also

• Dark quencher