COMPLEAT (bioinformatics tool)

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Protein Complex Enrichment Analysis Tool is an online bioinformatics tool used to analyze high-throughput datasets (or small-scale datasets) using protein complex enrichment analysis.[1] The tool uses a protein complex resource as the back end annotation data instead of conventional gene ontology- or pathway-based annotations. The tool incorporates several useful features in order to provide a comprehensive data-mining environment, including network-based visualization and interactive querying options.

COMPLEAT may be used to analyze RNAi screens, proteomic datasets, gene expression data and any other high-throughput datasets where protein complex information is relevant.

Applications

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COMPLEAT has been successfully applied to identify:

  • Dynamic protein complexes regulated by insulin and epidermal growth factors signaling, including a role of Brahma complex in the cellular response to insulin.[1]
  • Evolutionarily conserved molecular complexes that regulate nucleolar size when the complex constituents were targeted by RNA interference.[2]
  • Novel role of endocytosis and vesicle trafficking complexes in Hippo Signaling Pathway.[3]

References

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