Tryptic soy broth
 | This article needs additional citations for verification. (April 2015) |
Tryptic soy broth or Trypticase soy broth (frequently abbreviated as TSB) is used in microbiology laboratories as a culture broth to grow aerobic and facultative anaerobic bacteria. It is a general purpose medium that is routinely used to grow bacteria which tend to have high nutritional requirements (i.e., they are fastidious).[1]
Uses
[edit | edit source]Sterility test medium in USP and EP as well as for inocula preparation for CLSI standards.[1]
TSB is frequently used in commercial diagnostics in conjunction with the additive sodium thioglycolate which promotes growth of anaerobes.[2]
Preparation
[edit | edit source]To prepare 1 liter of TSB, the following ingredients are dissolved under gentle heat. Adjustments to pH should be made using 1N HCl or 1N NaOH to reach a final target pH of 7.3 ± 0.2 at 25°C. The solution is then autoclaved for 15 minutes at 121°C.[3]
Tryptic Soy Agar contains per liter:
- 17 g pancreatic digest of casein
- 3 g peptic digest of soybean
- 5 g sodium chloride
- 2.5g dipotassium phosphate (K2HPO4)
- 2.5g glucose
References
[edit | edit source]- ^ a b Lua error in Module:Citation/CS1/Configuration at line 2172: attempt to index field '?' (a nil value).
- ^ Lua error in Module:Citation/CS1/Configuration at line 2172: attempt to index field '?' (a nil value).
- ^ Lua error in Module:Citation/CS1/Configuration at line 2172: attempt to index field '?' (a nil value).[dead link]
 | This microbiology-related article is a stub. You can help Wikipedia by expanding it. |