R2A agar
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R2A agar (Reasoner's 2A agar) is a culture medium[1] developed to study bacteria which normally inhabit potable water.[2] These bacteria tend to be slow-growing species and would quickly be suppressed by faster-growing species on a richer culture medium.[3]
Since its development in 1985,[3] it has been found to allow the culturing of many other bacteria that will not readily grow on fuller, complex organic media.[citation needed]
Typical composition (% w/v)
[edit | edit source]- Proteose peptone, 0.05%
- Casamino acids, 0.05%
- Yeast extract, 0.05%
- Dextrose, 0.05%
- Soluble starch, 0.05%
- Dipotassium phosphate, 0.03%
- Magnesium sulfate, 0.005%
- Sodium pyruvate, 0.03%
- Agar, 1.5%
Final pH 7.2 ± 0.2 @ 25 °C
References
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