Limulus clotting factor C

Limulus clotting factor overbar C
Identifiers
EC no.	3.4.21.84
CAS no.	115743-27-6
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

Limulus clotting factor C
Identifiers
Organism	Tachypleus tridentatus
Symbol	?
UniProt	P28175
Search for Structures Swiss-model Domains InterPro

Limulus clotting factor overbar C (EC 3.4.21.84, factor C, limulus factor C) is an enzyme. This enzyme catalyses the following chemical reaction

Selective cleavage of -Arg¹⁰³-Ser- and -Ile¹²⁴-Ile- bonds in limulus clotting factor B to form factor overbar B.

Cleavage of -Pro-Arg- bonds in synthetic substrates

This enzyme is isolated from the hemocyte granules of the horseshoe crabs Limulus and Tachypleus, where it serves as a LPS endotoxin-sensitive trypsin type serine protease to protect the organism from bacterial infection, initiating a cascade leading to coagulin formation. From the N-terminus to the C-terminus, the domains are:

• EGF-like domain
• 3 Sushi domains
• one LCCL domain and one C-type lectin domain
• 2 more Sushi domains
• a trypsin domain

This enzyme is useful in Limulus amebocyte lysate as the endotoxin-detecting element. It can be produced recombinantly.

External links

• Limulus+clotting+factor+_overbar_C_ at the U.S. National Library of Medicine Medical Subject Headings (MeSH)