Nucleic acid methods
(Redirected from DNA purification)
Nucleic acid methods are the techniques used to study nucleic acids: DNA and RNA.
Purification
[edit | edit source]- DNA extraction[1]
- Phenol–chloroform extraction
- Minicolumn purification
- RNA extraction
- Boom method
- Synchronous coefficient of drag alteration (SCODA) DNA purification
Quantification
[edit | edit source]- Abundance in weight: spectroscopic nucleic acid quantitation
- Absolute abundance in number: real-time polymerase chain reaction (quantitative PCR)
- High-throughput relative abundance: DNA microarray
- High-throughput absolute abundance: serial analysis of gene expression (SAGE)
- Size: gel electrophoresis
Synthesis
[edit | edit source]- De novo: oligonucleotide synthesis
- Amplification: polymerase chain reaction (PCR), loop-mediated isothermal amplification (LAMP), transcription-mediated amplification (TMA)
Kinetics
[edit | edit source]- Multi-parametric surface plasmon resonance[2][3]
- Dual-polarization interferometry[4]
- Quartz crystal microbalance with dissipation monitoring (QCM-D)[5]
Gene function
[edit | edit source]Other
[edit | edit source]- Bisulfite sequencing
- DNA sequencing
- Expression cloning
- Fluorescence in situ hybridization
- Lab-on-a-chip
- Comparison of nucleic acid simulation software
- Northern blot
- Nuclear run-on assay
- Radioactivity in the life sciences
- Southern blot
- Differential centrifugation (sucrose gradient)
- Toeprinting assay
- Several bioinformatics methods, as seen in list of RNA structure prediction software
See also
[edit | edit source]References
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